De Groot, a three-year professional from Coffs Harbour in New South Wales, carded an opening 71, one-under-par, to sit in a tie for 11th after the first round.
She has been playing on the secondary Symetra Tour in the United States, after attending college.
Brogan McKinnon of Canada carded an opening 66 to lead the field in the four-round second stage of qualifying. Of the 192 players in the field, the top-80 advance to final stage of Qualifying Tournament from December 3-7 in Daytona Beach, Florida.
Lee and Oh, both 18, shot even-par 72s to sit in equal-28th position along with Lauren Hibbert, of New South Wales, who is the touring professional at Asquith Golf Club near Sydney.
AUSTRALIAN SCORES
71 Emma De Groot
72 Su Oh (a), Minjee Lee, Lauren Hibbert
74 Maggie Yuan
76 Ellen Davies-Graham
77 Bree Elliott, Jayde Panos (a)
78 Justine Lee (a)
81 Stephanie Na